LAMP (Loop mediated isothermal amplification) em diferentes proporções de DNA equino e bovino

Abstract

Meat adulteration has become an issue of global concern. Existing methods for identifying equine contamination are expensive and complex, requiring specialized equipment. We developed a specific, quick and easy to visualize method: LAMP, which in just 1 hour detects up to 0.1% of equine meat. Through this technique, it is possible to detect equine DNA using different proportions of equine DNA from meat samples, as well as identify the sensitivity level of LAMP, that is, identify the proportion between equine and bovine DNA that LAMP can detect. The result can be observed with the naked eye, through intercalating dyes. The technique stands out for being isothermal, simple and fast, and can be performed even in the simplest laboratories.