Seleção de bactérias promotoras de crescimento para solubilização de fósforo, zinco e produção de amilase

Abstract

This course conclusion work discusses plant growth-promoting microorganisms (MPCVs) for the solubilization of phosphorus and zinc, as well as the production of amylase. MPCVs are microorganisms that can colonize the rhizosphere of plants, promoting their growth through several mechanisms, including the solubilization of essential nutrients. In the study, different bacterial isolates were analyzed for their ability to solubilize phosphorus and zinc, in addition to producing amylolytic enzymes. The bacterial isolates were tested in a specific culture medium for each nutrient. Analysis of variance (ANOVA) was used to determine the statistical significance of the results, followed by the Scott-Knott test for multiple comparisons. The results indicated that some isolates, such as AGL 14, showed high phosphorus solubilization capacity, while others, such as AGL 26, showed low or no activity. Analysis of the zinc solubilization indices of bacterial isolates revealed a wide variation in their capabilities, the average zinc solubilization halo diameters for AGL 2, AGL 9 and AGL 4 highlight the ability of these strains to form clear zones around colonies . The data obtained from amylase activity revealed a significant variation among the bacterial isolates tested. Amylase activity, measured by the diameter of the degradation halo around the colonies, is indicative of the strains' ability to hydrolyze the starch present in the culture medium.