Teste de viabilidade de acesso para Xanthomonas spp. E Colletotrichum Lindemuthianum submetidos ao processo de liofilização

Abstract

Xanthomonas spp. and Colletotrichum lindemuthianum highlight the urgent need for effective long-term conservation methods in the face of constantly evolving phytopathogenic threats. Such strategies are crucial to preserve the viability and integrity of these microorganisms, fostering advancements in agricultural research to address challenges in economically significant crops. This study investigates the post-lyophilization viability of Xanthomonas spp. and C. lindemuthianum, focusing on the survival and maintenance of original cellular characteristics. Six cryoprotectors (sodium chloride, sucrose, fructose, lactose, maltodextrin, and mannitol 59 mol) were tested. Twenty-four isolates (twelve of each species) underwent examination with different storage periods (Time I - 0 days, Time II - 15 days, Time III - 40 days). Results indicated maintained viability for Xanthomonas spp., with a slight growth delay using fructose as a cryoprotector. C. lindemuthianum exhibited satisfactory lyophilization results, except with sodium chloride as a cryoprotector, causing growth disparities, possibly due to high salinity.