Avaliação do potencial mutagênico do extrato de brosimum gaudichaudii (mama-cadela)

Abstract

ABSTRACT The (Brosimum gaudichaudii) popularly known as: mama-cadela, is a lactescent shrub quite common in folk medicine for the treatment and combat of diseases related to the skin. The objective of this work is to evaluate the mutagenic activities of different concentrations of Mama-cadela (Brosimum gaudichaudii) extracts by the Allium cepa test. For the experiment, 06 roots of the onion bulbs were removed and placed in beakers with fresh tap water for rooting the bulbs at room temperature. Subsequently, 06 roots were collected, without exposure to the extract of Mama-Cadela, being considered as a negative control group. The other roots were exposed for 24 hours in extractive solutions at 3 different concentrations, corresponding to 1g/L, 5g/L and 10g/L. After 24 hours of exposure, the rest of the roots exposed to the treatments were removed in order to evaluate the cellular changes induced by the exposure to the extract. The analysis of the slides was performed randomly, without prior knowledge of the treatments, using white light microscopy with a 40X objective. 1000 interphase cells were analyzed for each treatment, totaling 3000 cells for each of the extractive samples. For the evaluation of the effects associated with exposure to the Mama-Cadela (Brosimum gaudichaudii) extract, the data obtained were tabulated in the Excel program (Microsoft office® 2016) and expressed as mean and standard deviation in percentage (frequency). When analyzing the cytotoxic and mutagenic potential of the plant extract through the analysis of the mitotic index and chromosome migration and other changes in apical root meristematic cells of Allium cepa, significant differences were observed when the concentrations of 0.1g/L were compared with the control. However, no significant values were observed for mitotic index and micronucleus and binicleate cell counts.